Description
Accession
P42203
Source
Optimized DNA sequence encodingrat Interleukin-13 mature chain was expressed in Escherichia Coli.
Molecular weight
NativeRat IL-13, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately12 kDa. Recombinant Rat IL-13 is a monomer protein consisting of111 amino acid residue subunits,and migrates as an approximately kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.0 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence
MALWVTAVLA LACLGGLATP GPVRRSTSPP VALRELIEEL SNITQDQKTS LCNSSMVWSV DLTAGGFCAA LESLTNISSC NAIHRTQRIL NGLCNQKASD VASSPPDTKI EVAQFISKLL NYSKQLFRYG H
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse IL-13 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
