Human Interleukin-5 Recombinant

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$70.00$4,700.00

SKU: RKP05113 Tags: , , , ,

Description

Accession
P05113
Source
Optimized DNA sequence encoding Human Interleukin-5 mature chain was expressed in Escherichia Coli.
Molecular weight
Nativehuman Interleukin-5, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa. Recombinant IL-5 is a disulfide-linked homodimeric protein consisting of two 116 amino acid residue subunits,and migrates as an approximately 26 kDa protein under non-reducing conditions and as a 13 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of TF-1 cells was ≤.2 ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence
MRMLLHLSLL ALGAAYVYAI PTEIPTSALV KETLALLSTH RTLLIANETL RIPVPVHKNH QLCTEEIFQG IGTLESQTVQ GGTVERLFKN LSLIKKYIDG QKKKCGEERR RVNQFLDYLQ EFLGVMNTEW IIES
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Interleukin-5 was lyophilized from a 0.2 μm filtered PBS solution pH7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Molecular function
Molecular function

Methods

Functional SMEZ is not required for production of inflammatory cytokines in superantigen-sensitive mice.

  • In contrast, GAS-M3

Isolation of PB and BM Eos

  • PB Eos were isolated using anti-CD16-conjugated magnetic beads following the manufacturer's protocol.
  • Briefly, blood granulocytes were recovered from whole blood via Percoll density centrifugation.
  • Subsequent incubation of these granulocytes with anti-CD16-conjugated magnetic beads for negative selection led to the retention of CD16+ neutrophils in a MACS column and recovery of untouched, CD16 PB eosinophils.
  • BM Eos were purified from aspirates as described previously.in vitro 8-day culture in a pro-Eos-survival medium (RPMIeos) containing 1 ng/ml recombinant IL-5 .
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