Human Interleukin-9 Recombinant
Categories: HematopoietinsIL-2 familyRecombinant Human Cytokines$70.00 – $4,700.00
Description
Accession
P15248
Source
Optimized DNA sequence encoding human Interleukin-9 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Interleukin-9 is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 14kDa. Recombinant human Interleukin-9 is a monomer protein consisting of 127 amino acid residue subunits, and migrates as an approximately 14 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent proliferation of human MO7e cells is <.4 ng/ml.
Protein Sequence
MLLAMVLTSA LLLCSVAGQG CPTLAGILDI NFLINKMQED PASKCHCSAN VTSCLCLGIP SDNCTRPCFS ERLSQMTNTT MQTRYPLIFS RVKKSVEVLK NNKCPYFSCE QPCNQTTAGN ALTFLKSLLE IFQKEKMRGM RGKI
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant human Interleukin-9 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q01113
Molecular function
Molecular function
Methods
Plasma levels of IL-9, -17, and -4 in Dengue patients and control groups.
- Plasma levels of IL-9 , -17 , and -4 were examined for Dengue patients (DF, DHF and DSS) and control groups (febrile illness and healthy subjects) by ELISA.
