Mouse IL-18 Recombinant

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$70.00$4,700.00

SKU: RKP70380 Tags: , , , , , ,

Description

Accession
P70380
Source
Optimized DNA sequence encoding mouse Interleukin-18 mature chain was expressed in E.Coli.
Molecular weight
Native mouse IL-18, generated by the proteolytic removal of the signal peptide and propeptide,and has a calculated molecular mass of approximately 18 kDa. Recombinant mouse IL-18 is a monomer protein consisting of 158 amino acid residue subunits, and migrates as an approximately 18 kDa protein under reducing conditions in SDS-PAGE.
Purity
>90%, as determined by SDS-PAGE and HPLC
Protein Sequence
MAAMSEDSCV NFKEMMFIDN TLYFIPEENG DLESDNFGRL HCTTAVIRNI NDQVLFVDKR QPVFEDMTDI DQSASEPQTR LIIYMYKDSE VRGLAVTLSV KDSKMSTLSC KNKIISFEEM DPPENIDDIQ SDLIFFQKRV PGHNKMEFES SLYEGHFLAC QKEDDAFKLI LKKKDENGDK SVMFTLTNLH QS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant mouse Interleukin-18 was is presented as lyophilized.2 μm filtered solution in PBS pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
P29466
Molecular function

Methods

PLZF regulates the expression of receptors for IL12 and IL18.

  • CD25 expression by wt and PLZF-deficient cells in response to IL-12 or anti-CD3 treatment.
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