Optimized DNA sequence encoding Mouse LIF mature chain was expressed in Escherichia Coli.
Native Mouse LIF is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 20kDa. LIF is a monomer protein consisting of 181 amino acid residue subunits, and migrates as an approximately 20kDa protein under reducing conditions in SDS-PAGE.
>98%, as determined by SDS-PAGE and HPLC.
The ED(50) was determined by the dose-dependent differentiation of M1 myeloid leukemic cells was found to be in the range of.01 ng/ml.
MKVLAAGIVP LLLLVLHWKH GAGSPLPITP VNATCAIRHP CHGNLMNQIK NQLAQLNGSA NALFISYYTA QGEPFPNNVE KLCAPNMTDF PSFHGNGTEK TKLVELYRMV AYLSASLTNI TRDQKVLNPT AVSLQVKLNA TIDVMRGLLS NVLCRLCNKY RVGHVDVPPV PDHSDKEAFQ RKKLGCQLLG TYKQVISVVV QAF
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
RecombinantLeukemia Inhibitory Factorwas lyophilized from a.2 μm filtered PBS pH.5.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.