2019 nCoV/COVID19 Nucleocapsid Recombinant-His
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$350.00 – $1,200.00
Description
Accession
NCOVNP
Source
DNA sequence encoding the COVID-19 Nucleocapsid protein domain ,amino acids[1-419] (accession# YP_009724397.2) including a C-terminal His tag was expressed in CHO Cells.
Molecular weight
Recombinant 2019-nCoV Nucleocapsid protein is a protein consisting of 431 amino acid residues ,due to glycosylation migrates as an approximately 70 kDa protein on SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant nCoV-2019 Nucleocapsid protein was lyophilized from 0.2 µm filtered PBS, pH 7.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
SARS COV-2 Nuclecapsid SDS PAGE
- The recombinant Nucleocapsid-His protein migrates as 70kDa due to glycosylation
SARS COV-2-NP Sandwich ELISA
- Recombinant SARS CoV2 NP-His protein (cat#RKNCOVNP) at varying concentrations 0.4-5 ng/well. Capture antibody: Anti NP Monoclonal (cat#RMANCOVNP1) Detection antibody:Anti NP Monoclonal (cat#RMANCOVNP2)
SARS COV-2-Nucleocapsid ELISA
- Recombinant SARS CoV2 NP-His protein (cat#RKNCOVNP) at 200ng/well. Primary antibody: Anti NP Monoclonal (cat#RMANCOVNP1) Secondary antibody:HRP Goat Anti rabbit IgG 1:10000
