DNA sequence encoding extracelluar domain of SARS Spike S protein [14-1255] accession#P59594 was including an C terminal PolyHis tag expressed CHO cells.
Recombinant SARS S . migrates as an approximately 140 kDa protein under and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE.
Lyophilized from PBS pH 7.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.