Description
Accession
P59594
Source
DNA sequence encoding extracelluar domain of SARS Spike protein [13-756] accession#Q0ZME7 was including an C terminal human IgG1 tag expressed CHO cells.
Molecular weight
Recombinant SARS S1 . migrates as an approximately 90 kDa protein under and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Biological Process
Biological Process
Methods
SARS (hcov) S1 Fc PAGE
- Tris-Bis PAGE under reducing condition. The purity is greater than 95%
SARS (hcov) Spike S1 Fc HPLC
- The purity of SARS hCov Spike S1 is greater than 95% as determined by SEC-HPLC.
SARS (hcov) Spike S1 Fc ELISA
- Functional ELISA showing immobilized Human ACE2 cat#RKQ9BYFH, the calculated EC50 was determined to be 0.4ug/ml .
