COV2 Nucleocapsid protein N B.1.1.529 (Omicron)-His
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$350.00 – $1,250.00
Description
Source
DNA sequence encoding extracellular fragment[1-419] of COV2 Nucleocapsid protein (B.1.1.529/Omicron) variant (P13L, E31 R32S33 deletion , R203K, G204R)including a C terminal polyHis tag was expressed in HEK cells.
Molecular weight
Recombinant NCOV nucleocapsid protein B.1.1.529 encodes the COV2 Nuclecapsid glycoprotein . Recombinant COV2 N protein migrate as an approximately 70 kDa protein under reducing conditions in SDS-PAGE.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant COVID-19 nuclecapsid was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
SARS COV-2 B.1.1.529 Nuclecapsid SDS PAGE
- The recombinant Nucleocapsid-His protein migrates as 70kDa due to glycosylation
SARS COV2 Nucleocapsid B.1.1.529 (omicron) HPLC
- The purity of 2019-nCoV B.1.1.529 nucleocpasid is greater than 95% as determined by SEC-HPLC.
