Anti SARS-COV2-Nucleocapsid Monoclonal Antibody 5B2
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$300.00 – $1,200.00
Description
Accession
RKNCOVSNP
Applications
ELISA: This Antibody was validated by ELISA binding to recombinant SARS CoV2 Nucleocapsid (cat#RKNCOVNP) (see image)
Source
The antibody was produced in rabbits immunized with recombinant SARS COV2 Nucleocapsid protein.
Species reactivity
Specific for both SARS CoV2 NP and COV1 NP, has not been tested against other viruses.
Isotype
Rabbit IgG
Purification
The IgG fraction of the tissue culture supernatant was purified by Protein A affinity chromatography.
Presentation
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS with 5% trehalose.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
This antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
SARS COV-2-NP ELISA
- Recombinant SARS CoV2 NP-His protein (cat#RKNCOVNP) at 200ng/well. Primary antibody: Anti NP Monoclonal (cat#RMANCOVNP1) Secondary antibody:HRP Goat Anti rabbit IgG 1:10000
SARS COV-2-NP Sandwich ELISA
- Recombinant SARS CoV2 NP-His protein (cat#RKNCOVNP) at varying concentrations 0.4-5 ng/well. Capture antibody: Anti NP Monoclonal (cat#RMANCOVNP1) Detection antibody:Anti NP Monoclonal (cat#RMANCOVNP2)
