2019 nCoV/COVID19 Spike protein S1 Recombinant ,Biotinylated
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$1,300.00 – $3,100.00
Description
Accession
NCOVS1
Source
DNA sequence encoding the COVID-19 Spike S1 protein extracellular domain,[16-683] (accession # QHD43416.1) fused to aq C-terminal His AVI tag was expressed in HEK Cells.
Biotinylation
The COV2 S1 is biotinylated in vitro at a single site using the AVItagTM biotinylation technology.
Activity
The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below).
Molecular weight
Recombinant 2019-nCoV Spike S1 is a protein consisting of 680 amino acid residues with a calculated mW of 78kDa ,due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated SARS COV2 S1 -human ACE functional binding ELISA assay
- Biotinylated COV2 S1 coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The EC50 was determined to be 35 ng/ml
Biotinylated S1 protein PAGE
- 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
SARS COV-2 Spike S1 Biotinylated HPLC
- The purity of 2019-nCoV Spike S1 is greater than 95% as determined by SEC-HPLC.
