COV2 Spike protein RBD Recombinant B.1.1.529 (Omicron)-His
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$350.00 – $1,200.00
Description
Accession
NCOVS1
Source
DNA sequence encoding extracellular fragment[319-541] of COV2 Spike RBD domain [accession# QIC53204.1] variant (B.1.1.529/Omicron) ( G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)including a C terminal polyHis tag was expressed in HEK cells.
Molecular weight
Recombinant COV2 RBD B.1.1.529 encodes the NCOV spike glycoprotein RBD domain sequence . This molecule has a calculated mass of approximately 76kDa. Recombinant COV2 RBD migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE.
Activity
The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below).
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant COVID-19 Spike RBD was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
SARS COV-2 Spike RBD B.1.1.529 omicron HPLC
- The purity of 2019-nCoV Spike RBD B.1.1.529 omicron is greater than 95% as determined by SEC-HPLC.
RBD variant B.1.1.529 omicron protein PAGE
- 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
SARS COV2 RBD B.1.1.529/omicron ACE functional binding ELISA assay
- COV2 RBD (coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The linear range was found to be 100 ng/ml
