Rat Interleukin-6 Recombinant
Categories: HematopoietinsIL-6 gp130 familyRecombinant Rat Cytokines$70.00 – $4,700.00
Description
Accession
P20607
Source
Optimized DNA sequence encoding rat Interleukin-6 mature chain was expressed in Escherichia Coli.
Molecular weight
Native rat Interleukin-6 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 22kDa. Recombinant rat Interleukin-6 is a monomer protein consisting of 188 amino acid residue subunits, and migrates as an approximately 22kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkeyMBr-5 cells was found to be in the range of.0-40.0 ng/ml.
Protein Sequence
MKFLSARDFQ PVAFLGLMLL TATAFPTSQV RRGDFTEDTT HNRPVYTTSQ VGGLITYVLR EILEMRKELC NGNSDCMNSD DALSENNLKL PEIQRNDGCF QTGYNQEICL LKICSGLLEF RFYLEFVKNN LQDNKKDKAR VIQSNTETLV HIFKQEIKDS YKIVLPTPTS NALLMEKLES QKEWLRTKTI QLILKALEEF LKVTMRSTRQ T
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant rat Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Molecular function
Methods
Chemicals and Kits
- The biochemical tests conducted with the specific enzymatic colorimetric assay kits were all provided by, either located at Switzerland or .
- The reagents IFCC and P-5-P were used for assay of GOT and GPT.
- Octacalcium phosphate (OCP) and UV were used for the determination of serum calcium and phosphate ions.
- For other determinations we used BCG for serum albumin, CHOD for cholesterol, lipase-glycerol oxidase for triglycerides; KINETIC for serum BUN, picric acid for creatinine (reaction), colorimetric oxidase for uric acid, and Sirius Red for collagen staining.
- Doxorubicin (DR) was a product of Pfizer .
- The kits for other determinations included SO and TBAS from Cayman (Michigan, ), the rat IL-6 EIA Kit from , and the rat TNF-α Kit from the& .
- The sources of the antibodies used in this experiment were: PDGF Receptor β (1∶1000), phosphor-PDGF Receptor β (1∶1000) and β-actin from Cell Signaling ; phosphor-PI3K (1∶500) from Santa…
2.5. Primary splenocyte and bone marrow-derived dendritic cell (BMDC) culture
- Untreated 6-week-old SD rats were sacrificed by cervical dislocation after ether exposure.
- Cells in the spleen were harvested and prepared as a single cell suspension in complete medium in a 24-well plate (1×107 cells/well).
- In the control group, splenocytes were incubated alone or with recombinant human TGF-ß (2 ng/ml, , , ) and recombinant rat IL-6 (20 ng/ml, , , ) at 37°C for 72 h. For the experimental groups, in addition to TGF-ß and IL-6, cells were treated with culture supernatant of
F. - prausnitzii, sodium butyrate (0.05834 µmol/well), denatured
F. - prausnitzii and
B. - longum bacteria (1× 107 CFU/well).
- Each group treatment was repeated four times.
- After 72 h, the supernatant of cultured splenocytes was collected and stored at −20°C for further analysis
