Biotinylated Human TNF alpha Protein Recombinant
Categories: Biotinylated cytokinesBiotinylated proteins$542.00 – $1,805.00
Description
Accession
P01375
Source
DNA sequence encoding Human TNF alpha including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human TNF alpha is a protein consisting of amino acid residues [Val77-Leu233] ,The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-24 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TNF alpha was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O88351
Interactor
O88522
Interactor
O89110
Interactor
Interactor
Interactor
Interactor
P39429
Interactor
P62991
Interactor
Q12933
Interactor
Q15628
Interactor
Q60680
Interactor
Q60769
Interactor
Q60855
Interactor
Q62073
Interactor
Q924T7
Interactor
Q9QZL0
Interactor
Interactor
Interactor
Interactor
Interactor
P70191
Interactor
Q13546
Interactor
Q3U0V2
Interactor
Q80TQ2
Interactor
Q9DHW0
Interactor
Q9QZL0
Molecular function
Methods
Biotinylated Human TNF alpha Protein Tris-Bis PAGE
- Biotinylated Human TNF alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human TNF alpha Protein SEC-HPLC
- The purity of Biotinylated Human TNF alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TNF alpha Protein ELISA Data
- Immobilized Human TNF R2, mFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 3.5ng/ml determined by ELISA.
