Optimized DNA sequence encoding extracellular domain of Human Low density lipoprotein receptor (ALA22-ARG788)including a C-terminal His tag was expressed in HEK293 cells. Recombinant LDL receptor was further labled by Biotin for detection by avidin conjugated secondary probes.
RecombinantLDL receptor is a protein consisting of 773 amino acid residue subunits, due to glycosylation migrates as an approximately 100kDa(mature form) and 140kDa(immature form) protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant Human PCSK9 to bind biotinylated recombinant human LDL Receptor in ELISA. The ED50 was calculated to be 0.6ug/ml.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Low density lipoprotein receptor is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
RecombinantLDL receptor can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.