DNA sequence encoding Human MIP-1A mature chain was expressed in Escherichia Coli. MIP1A was further labeled by Biotin for detection by Avidin conjugated secondary probes
Native human MIP-1 alpha is generated by the proteolytic removal of the signal peptide.This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant MIP-1 alpha is a monomer protein consisting of 70 amino acid residue subunits, andmigrates as an approximately8 kDa protein under non-reducing andreducing conditions in SDS-PAGE.
>98%, as determined by SDS-PAGE and HPLC
Biotinylated MIP-1A was designed to be used in single step detection in ELISA ,WB
MQVSTAALAV LLCTMALCNQ FSASLAADTP TACCFSYTSR QIPQNFIADY FETSSQCSKP GVIFLTKRSR QVCADPSEEW VQKYVSDLEL SA
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant MIP-1 was lyophilized from a.2 μm filtered PBS solution pH7.5.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.