Optimized DNA sequence encoding extracellular domain of Human Programmed cell death protein 1 PD-1 (PRO21 - GLN167) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human PD-1 (PDCD1) is a protein consisting of 150 amino acid residue subunits,due to glycosylation migrates as an approximately 40kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant Human PD-1 to bind recombinant human PD-L1 within a linear range of 0.1-5.0 ng/ml in ELISA.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human PD-1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in sterile PBS pH 7.4 to a concentration not less than 0.2 mg/mL. Solubilize for 1hr at room temp with gentle mixing.
Recombinant PD-1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months.It is recommended to add 0.1% HSA or BSA for long term storage. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.