Human basic FGF Recombinant, Biotinylated
Categories: Biotinylated proteinsCytokinesFGF familyFGF familyGrowth FactorsRecombinant Human Cytokines$340.00 – $1,950.00
Description
Accession
P09038
Source
Optimized DNA sequence encoding Human basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight
Native human FGF basic is generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated mass of kDa. Recombinant bFGF is a monomer protein consisting of 155 amino acid residue subunits, and migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Applications
ELISA,WB Biotinylated FGF basic was designed to be used in single step detection in ELISA ,WB
Protein Sequence
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Human Basic FGF was lyophilized from a 0.2 μm filtered solution inM NaCl,20mM PB pH7.0 .
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O35082
Interactor
O43353
Interactor
Interactor
Interactor
Q802A9
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function