Optimized DNA sequence encoding peptidase domain of Human R-Spondin1(SER21-ALA263 ) including a C-terminal His tag was expressed in HEK293 cells. Recombinant R-spondin1 was further labled by Biotin for detection by avidin conjugated secondary probes.
Recombinant human R-Spondin-1 is a protein consisting of 433 amino acid residue subunits,due to glycosylation migrates as an approximately 53kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
ELISA,WB Biotinylated recombinant R-spondin1 was designed to be used in single step detection in ELISA ,WB
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human R-spondin1 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
RecombinantR-spondin1can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.