Biotinylated Human Nectin Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q96NY8
Source
DNA sequence encoding Human Nectin-4 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Nectin-4 is a protein consisting of amino acid residues [Gly32-Val351] ,The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Nectin-4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Q786F2
Methods
Biotinylated Human Nectin-4 Protein Tris-Bis PAGE
- Biotinylated Human Nectin-4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human Nectin-4 Protein SEC-HPLC
- The purity of Bioyintlated Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Nectin-4 Protein ELISA Data
- Immobilized Biotinylated Human Nectin-4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
