/, Biotinylated proteins, Cytokines/Human Interleukin-4 Recombinant, Biotinylated

Human Interleukin-4 Recombinant, Biotinylated

Categories:

$160.00$4,700.00

SKU: RBP05112 Tags: , , , , , ,

Description

Accession
P05112
Source
DNA sequence encoding Human IL4 including a C-terminal AVI polyHis tag was expressed in HEK293 cells. Recombinant Human IL4 was further labeled by Biotin for detection by avidin conjugated secondary probes.
Molecular weight
Recombinant Human IL4 is a protein consisting of amino acid residues [Met23-Ser153] with a theoretical MW of 15kD. Recombinant IL-4 migrates as an approximately 20 kDa protein on SDS-PAGE due to glycosylation.
Bioactivity
The activity was tested by the ability to bind human IL4R in an ELISA assay at a linear range of 2-10 ng/ml.
Purity
>95%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant IL4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Sequence
MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Molecular function
Molecular function
Stay in the loop
For new product information and regular updates on our product range please fill your details
Loading