Human Interleukin-4 Recombinant, Biotinylated
Categories: Biotinylated proteinsCytokinesRecombinant Human Cytokines$160.00 – $4,700.00
Description
Accession
P05112
Source
DNA sequence encoding Human IL4 including a C-terminal AVI polyHis tag was expressed in HEK293 cells. Recombinant Human IL4 was further labeled by Biotin for detection by avidin conjugated secondary probes.
Molecular weight
Recombinant Human IL4 is a protein consisting of amino acid residues [Met23-Ser153] with a theoretical MW of 15kD. Recombinant IL-4 migrates as an approximately 20 kDa protein on SDS-PAGE due to glycosylation.
Bioactivity
The activity was tested by the ability to bind human IL4R in an ELISA assay at a linear range of 2-10 ng/ml.
Purity
>95%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant IL4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Sequence
MGLTSQLLPP LFFLLACAGN FVHGHKCDIT LQEIIKTLNS LTEQKTLCTE LTVTDIFAAS KNTTEKETFC RAATVLRQFY SHHEKDTRCL GATAQQFHRH KQLIRFLKRL DRNLWGLAGL NSCPVKEANQ STLENFLERL KTIMREKYSK
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Molecular function
Molecular function