DNA sequence encoding extracellular domain of Human HER3 (ERBB3) to a C-terminal polyHis tag was expressed in CHO cells.
Recombinant Human HER3/ERBB3 is a monomer consisting of 640 amino acid residue , and migrates as an approximately 75 kDa protein under reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE
ELISA,WB: Biotinylated HER3 was designed to be used in single step detection in ELISA ,WB
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human HER2 was lyophilized from a 0.2 μm filtered PBS pH 7.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.