Biotinylated Human CTLA/CD152 Protein Recombinant
Category: Biotinylated proteins$309.00 – $1,031.00
Description
Accession
P16410
Source
DNA sequence encoding Human CTLA-4/CD152 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CTLA-4/CD152 is a protein consisting of amino acid residues [Lys36-Asp161] ,The protein has a predicted MW of 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CTLA-4/CD152 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Methods
Biotinylated Human CTLA-4/CD152 Protein Tris-Bis PAGE
- Biotinylated Human CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human CTLA-4/CD152 Protein SEC-HPLC
- The purity of Biotinylated Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CTLA-4/CD152 Protein ELISA Data
- Immobilized Biotinylated Human CTLA-4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.
Biotinylated Human CTLA-4/CD152 Protein
- Immobilized Human B7-1, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 0.28μg/ml determined by ELISA.
