Biotinylated Human BTLA Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q7Z6A9
Source
DNA sequence encoding Human BTLA including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human BTLA is a protein consisting of amino acid residues [Lys31-Ser150] ,The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human BTLA was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Molecular function
Methods
Biotinylated Human BTLA Protein Tris-Bis PAGE
- Biotinylated Human BTLAÂ on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human BTLA Protein SEC-HPLC
- The purity of Biotinylated Human BTLA is greater than 95% as determined by SEC-HPLC
Biotinylated Human BTLA Protein ELISA Data
- Immobilized Anti-BTLA Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BTLA, His Tag with the EC50 of 8.0ng/ml determined by ELISA.