Biotinylated Cynomolgus MSLN/Mesothelin Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
A0A2K5TW94
Source
DNA sequence encoding Cynomolgus MSLN/Mesothelin (Primary Amine Labeling) including a C-His tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Cynomolgus MSLN/Mesothelin (Primary Amine Labeling) is a protein consisting of amino acid residues [Asp296-Gly580] ,The protein has a predicted MW of 33 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus MSLN/Mesothelin (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) Tris-Bis PAGE
- Biotinylated Cynomolgus MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) SEC-HPLC
- The purity of Biotinylated Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus MSLN/Mesothelin Protein (Primary Amine Labeling) ELISA Data
- Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Cynomolgus MSLN, His Tag with the EC50 of 54.8ng/ml determined by ELISA.
