Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein Recombinant
Category: Biotinylated proteins$696.00 – $2,320.00
Description
Accession
A0A2K5X2X9
Source
DNA sequence encoding Cynomolgus GARP&Latent TGF beta 1 Complex including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Cynomolgus GARP&Latent TGF beta 1 Complex is a protein consisting of amino acid residues [Ala18-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)] ,The protein has a predicted MW of 70.5 kDa (GARP)&41.3 kDa (Latent TGF Beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&13 kDa&42-50 kDa (Latent TGF Beta) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Cynomolgus GARP&Latent TGF beta 1 Complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein Tris-Bis PAGE
- Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein SEC-HPLC
- The purity of Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex Protein ELISA Data
- Immobilized Anti-GARP&Latent TGF beta 1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.38μg/ml determined by ELISA.
