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Sheep Basic Fibroblast Growth Factor Recombinant
Categories: FGF familyFood grade growth factorsSheep growth factors$120.00 – $500.00
Description
Accession
P20003
Source
Optimized DNA sequence encoding Sheep/Ovine Basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight
Native Ovine/Sheep bFGF is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 17kDa. Recombinant Ovine/Sheep basic FGF is a monomer protein, consisting of 155 amino acids and migrates as an approximately 17kDa protein under reducing conditions.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the proliferation of NR6R-3T3 fibroblasts, was found to be less than 0.1 ng/ml,corresponding to 1x10^6 IU/mg.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Ovine bFGF was lyophilized from a 0.2 μm filtered PBS solution pH7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
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