Human acidic Fibroblast Growth Factor Recombinant
Categories: FGF familyFGF familyRecombinant Human Cytokines$70.00 – $1,350.00
Description
Accession
P05230
Source
Optimized DNA sequence encoding HumanacidicFibroblast Growth Factor mature chain was expressed in Escherichia Coli.
Molecular weight
Native human aFGF, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated mass of kDa. Recombinant FGF acidicis a monomer protein consisting of amino acidsandmigrates as an approximately16 kDa protein under reducing and non-reducing conditions.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED50 as determined by thedose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ ng/ml, corresponding to a specific activity of ≥ x units/mg.
Protein Sequence
MAEGEITTFT ALTEKFNLPP GNYKKPKLLY CSNGGHFLRI LPDGTVDGTR DRSDQHIQLQ LSAESVGEVY IKSTETGQYL AMDTDGLLYG SQTPNEECLF LERLEENHYN TYISKKHAEK NWFVGLKKNG SCKRGPRTHY GQKAILFLPL PVSSD
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant acidic FGFwas lyophilized from a 0.2 μm filtered solution in.5% glycine,.5% sucrose,.01% Tween80, mM Glutamic acid, pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Interactor
Q8NI16
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
R1MAb2 activates FGFR1 in the presence of Klotho.
- Transfected cells were incubated with increasing concentrations of R1MAb2, human FGF23, or a positive control, FGF1, for 8 h before luciferase assays.
