Optimized DNA sequence encoding mouse CCL25 mature chain was expressed in Escherichia Coli.
Native mouse CCL25 has a calculated molecular mass of approximately 14 kDa. Recombinant CCL25 is a monomer protein consisting of 121 amino acid residue subunits, and migrates as an approximately 14 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Activitywas determined by the ability to induce chemotaxis of purified human monocytes at concentrations ranging between-10 ng/ml.
MKLWLFACLV ACFVGAWMPV VHAQGAFEDC CLGYQHRIKW NVLRHARNYH QQEVSGSCNL RAVRFYFRQK VVCGNPEDMN VKRAIRILTA RKRLVHWKSA SDSQTERKKS NHMKSKVENP NSTSVRSATL GHPRMVMMPR KTNN
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant CCL25 waswas lyophilized from.2μm filtered PBS , pH.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.