Description
Accession
P55773
Source
Optimized DNA sequence encoding Human MIP-3 (CCL23) mature chain was expressed in Escherichia Coli.
Molecular weight
Human CCL23, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately11kDa. Recombinant MIP-3(CCL-23) is a monomeric protein consisting of amino acid residue subunits and migrates as an approximately11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human T cells using a concentration range of.0-40.0 ng/ml.
Protein Sequence
MKVSVAALSC LMLVTALGSQ ARVTKDAETE FMMSKLPLEN PVLLDRFHAT SADCCISYTP RSIPCSLLES YFETNSECSK PGVIFLTKKG RRFCANPSDK QVQVCMRMLK LDTRIKTRKN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant CCL23 was lyophilized from a 0.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Molecular function
