Optimized DNA sequence encoding mouse MCP-2(CCL8) mature chain was expressed in Escherichia Coli.
Mouse MCP-2, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately9 kDa. RecombinantMCP-2 is a monomeric protein consisting of79 amino acid residue subunits and migrates as an approximately9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
>98%, as determined by SDS-PAGE and HPLC
The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of-100 ng/ml.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Mouse MCP-2 was lyophilized from a.2 μm filteredmM PB solution pH.5,mM NaCl.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.