Description
Accession
Q9Z121
Source
Optimized DNA sequence encoding mouse MCP-2(CCL8) mature chain was expressed in Escherichia Coli.
Molecular weight
Mouse MCP-2, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately9 kDa. RecombinantMCP-2 is a monomeric protein consisting of79 amino acid residue subunits and migrates as an approximately9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent chemo-attraction human PBMC using a range of-100 ng/ml.
Protein Sequence
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Mouse MCP-2 was lyophilized from a 0.2 μm filteredmM PB solution pH.5,mM NaCl.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Molecular function
Methods
Ccr1 ligands are induced after Candida infection and are chemotactic for kidney neutrophils ex vivo.
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