Optimized DNA sequence encoding Human Eotaxin-1 mature chain was expressed in Escherichia Coli.
Native human Eotaxin-1 has a calculated molecular mass of approximately 8 kDa. Recombinant Eotaxin-1 is a monomer protein consisting of 74 amino acid residue subunits, andmigrates as an approximately 8 kDa protein under non-reducingand reducing conditions in SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Activitywas determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between-100 ng/ml.
MKVSAALLWL LLIAAAFSPQ GLAGPASVPT TCCFNLANRK IPLQRLESYR RITSGKCPQK AVIFKTKLAK DICADPKKKW VQDSMKYLDQ KSPTPKP
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant Eotaxin-1 wasis lyophilized from as.2μm filtered concentrated (1.0mg/ml) PBS solution, pH.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.