Optimized DNA sequence encoding Mouse Interleukin-33 mature chain was expressed in Escherichia Coli.
Native Mouse Interleukin-33, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18kDa. Recombinant Interleukin-33 is a monomer protein consisting of 158 amino acid residue subunits,and migrates as an approximately 18kDa protein under non-reducing and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The ED(50) was determined by the dose-dependent proliferation ofmurine D10S cells is ≤.3 ng/ml.
MRPRMKYSNS KISPAKFSST AGEALVPPCK IRRSQQKTKE FCHVYCMRLR SGLTIRKETS YFRKEPTKRY SLKSGTKHEE NFSAYPRDSR KRSLLGSIQA FAASVDTLSI QGTSLLTQSP ASLSTYNDQS VSFVLENGCY VINVDDSGKD QEQDQVLLRY YESPCPASQS GDGVDGKKLM VNMSPIKDTD IWLHANDKDY SVELQRGDVS PPEQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE EKDESCNNIM FKLSKI
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant Interleukin-33 was lyophilized from a.2 μm filtered PBS solution pH7.5.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.