Optimized DNA sequence encoding Human CCL4/MIP-1 beta chain was expressed in Escherichia Coli.
Native human MIP-1 beta is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant CCL4/MIP-1 beta is a disulfide-linked homodimeric protein consisting of 69 amino acid residue subunits,and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Determined by its ability to chemoattract human blood monocytes using a concentration range of.0-10.0 ng/ml.
MKLCVTVLSL LMLVAAFCSP ALSAPMGSDP PTACCFSYTA RKLPRNFVVD YYETSSLCSQ PAVVFQTKRS KQVCADPSES WVQEYVYDLE LN
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant MIP-1 beta was lyophilized from a.2 μm filtered PBS solution.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
ELISA confirms CBMC antiviral chemokine release following antibody-enhanced dengue virus infection or polyI:C exposure.
- After 24 hours, cell supernatants were harvested and measured for CCL4, CCL5 and CXCL10 by ELISA.