/, CCL chemokines/Human MIP-1 beta (CCL4) Recombinant

Human MIP-1 beta (CCL4) Recombinant

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$70.00$2,700.00

SKU: RKP13236 Tags: , , , , , , , , , , , , ,

Description

Accession
P13236
Source
Optimized DNA sequence encoding Human CCL4/MIP-1 beta chain was expressed in Escherichia Coli.
Molecular weight
Native human MIP-1 beta is generated by the proteolytic removal of the signal peptide and propeptide. This molecule has a calculated molecular mass of approximately 8 kDa. Recombinant CCL4/MIP-1 beta is a disulfide-linked homodimeric protein consisting of 69 amino acid residue subunits,and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human blood monocytes using a concentration range of.0-10.0 ng/ml.

Protein Sequence
MKLCVTVLSL LMLVAAFCSP ALSAPMGSDP PTACCFSYTA RKLPRNFVVD YYETSSLCSQ PAVVFQTKRS KQVCADPSES WVQEYVYDLE LN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant MIP-1 beta was lyophilized from a.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P51677
Interactor
P32246
Interactor
P32302
Interactor
P51681
Biological Process
Biological Process
Molecular function

Methods

ELISA confirms CBMC antiviral chemokine release following antibody-enhanced dengue virus infection or polyI:C exposure.

  • After 24 hours, cell supernatants were harvested and measured for CCL4, CCL5 and CXCL10 by ELISA.

(A) Relative quantification of chemokine mRNA expression to GUS and IPO8 by tissue type.

  • Western blots probing for CCL3, CCL4 and GUS in eight samples of CRC with matched distal colon of standard protein concentration (2 mg BSA per ml).
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