Biotinylated SARS-COV-2 Spike S1 (Omicron B.529) Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
QHO60594
Source
DNA sequence encoding SARS-COV-2 Spike S1 (Omicron B.1.1.529) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) is a protein consisting of amino acid residues [Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)] ,The protein has a predicted MW of 77.94 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein Tris-Bis PAGE
- Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein SEC-HPLC
- The purity of Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) is greater than 95% as determined by SEC-HPLC.
Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein ELISA Data
- Immobilized Biotinylated Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.25μg/ml determined by ELISA.
