Biotinylated Human TSLP (R127A, R130A) Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q969D9
Source
DNA sequence encoding Human TSLP (R127A, R130A) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human TSLP (R127A, R130A) is a protein consisting of amino acid residues [Tyr29-Gln159(R127A,R130A)] ,The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TSLP (R127A, R130A) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Biotinylated Human TSLP (R127A, R130A) Protein Tris-Bis PAGE
- Biotinylated Human TSLP (R127A, R130A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human TSLP (R127A, R130A) Protein SEC-HPLC
- The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TSLP (R127A, R130A) Protein ELISA Data
- Immobilized Biotinylated Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA.
Biotinylated Human TSLP (R127A, R130A) Protein ELISA Data
- Immobilized Anti-TSLP Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.
