Biotinylated Human Oncostatin M/OSM Protein Recombinant
Categories: Biotinylated cytokinesBiotinylated proteins$542.00 – $1,805.00
Description
Accession
P13725
Source
DNA sequence encoding Human Oncostatin M/OSM including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Oncostatin M/OSM is a protein consisting of amino acid residues [Ala26-Arg221] ,The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Oncostatin M/OSM was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Biological Process
Molecular function
Molecular function
Methods
Biotinylated Human Oncostatin M/OSM Protein Tris-Bis PAGE
- Biotinylated Oncostatin M on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human Oncostatin M/OSM Protein SEC-HPLC
- The purity of Biotinylated Oncostatin M is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Oncostatin M/OSM Protein SPR Data
- Human LIF R, His Tag immobilized on CM5 Chip can bind Biotinylated Human Oncostatin M, His Tag with an affinity constant of 0.94 μM as determined in SPR assay (Biacore T200).
