Biotinylated Human LILRB2/CD85d/ILT4 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q8N423
Source
DNA sequence encoding Human LILRB2/CD85d/ILT4 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human LILRB2/CD85d/ILT4 is a protein consisting of amino acid residues [Gln22-His458] ,The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human LILRB2/CD85d/ILT4 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q86XS5
Interactor
Biological Process
Molecular function
Methods
Biotinylated Human LILRB2/CD85d/ILT4 Protein Tris-Bis PAGE
- Biotinylated Human LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human LILRB2/CD85d/ILT4 Protein SEC-HPLC
- The purity of Biotinylated Human LILRB2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human LILRB2/CD85d/ILT4 Protein ELISA Data
- Immobilized Anti-LILRB2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LILRB2, His Tag with the EC50 of 72.6ng/ml determined by ELISA.
