Biotinylated Human IL-18 Protein Recombinant
Categories: Biotinylated cytokinesBiotinylated proteins$542.00 – $1,805.00
Description
Accession
Q14116
Source
DNA sequence encoding Human IL-18 (Primary Amine Labeling) including a No Tag tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IL-18 (Primary Amine Labeling) is a protein consisting of amino acid residues [Tyr37-Asp193] ,The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-18 (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q9IW12
Interactor
Q99665
Molecular function
Methods
Biotinylated Human IL-18 Protein (Primary Amine Labeling) Tris-Bis PAGE
- Biotinylated Human IL-18 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human IL-18 Protein (Primary Amine Labeling) ELISA Data
- Immobilized Human IL-18BP, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 26.0ng/ml determined by ELISA.
