//Biotinylated Human IGF1R/CD221 Protein Recombinant

Biotinylated Human IGF1R/CD221 Protein Recombinant

Category:

$542.00$1,805.00

SKU: KT2SMEEHB

Description

Accession
P08069
Source
DNA sequence encoding Human IGF1R/CD221 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IGF1R/CD221 is a protein consisting of amino acid residues [Glu31-Asn932] ,The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IGF1R/CD221 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
P27986
Interactor
P29353
Interactor
P35570
Interactor
P81122
Interactor
Q14620
Interactor
Q06124
Molecular function
Molecular function

Methods

Biotinylated Human IGF1R/CD221 Protein Tris-Bis PAGE

  • Biotinylated Human IGF1R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human IGF1R/CD221 Protein SEC-HPLC

  • The purity of Biotinylated Human IGF1R is greater than 95% as determined by SEC-HPLC.

Biotinylated Human IGF1R/CD221 Protein ELISA Data

  • Immobilized Anti-IGF1R Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA.
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