Biotinylated Human DKK1 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
O94907
Source
DNA sequence encoding Human DKK1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human DKK1 is a protein consisting of amino acid residues [Thr32-His266] ,The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human DKK1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
O75581
Interactor
O88572
Interactor
Molecular function
Methods
Biotinylated Human DKK1 Protein Tris-Bis PAGE
- Biotinylated Human DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human DKK1 Protein SEC-HPLC
- The purity of Biotinylated Human DKK1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human DKK1 Protein ELISA Data
- Immobilized Biotinylated Human DKK1, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA.
Biotinylated Human DKK1 Protein
- Immobilized Anti-DKK1 Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 2.3ng/ml determined by ELISA.
