Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) Recombinant
Category: Biotinylated proteins$354.00 – $985.00
Description
Accession
P32970
Source
DNA sequence encoding Human CD27 Ligand/CD70 Trimer (Primary Amine Labeling) including a N-His tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD27 Ligand/CD70 Trimer (Primary Amine Labeling) is a protein consisting of amino acid residues [Leu50-Pro193] ,The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD27 Ligand/CD70 Trimer (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) Tris-Bis PAGE
- Biotinylated Human CD27 Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) SEC-HPLC
- The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) ELISA Data
- Immobilized Biotinylated Human CD27 Ligand (Trimer) , His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 67ng/ml determined by ELISA.
