//Biotinylated Human CD155/PVR Protein Recombinant

Biotinylated Human CD155/PVR Protein Recombinant

Category:

$542.00$1,805.00

SKU: KTWX22THB

Description

Accession
P15151
Source
DNA sequence encoding Human CD155/PVR including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD155/PVR is a protein consisting of amino acid residues [Trp21-Asn343] ,The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD155/PVR was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Biological Process
Biological Process
Molecular function

Methods

Biotinylated Human CD155/PVR Protein Tris-Bis PAGE

  • Biotinylated Human CD155 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Biotinylated Human CD155/PVR Protein SEC-HPLC

  • The purity of Biotinylated Human CD155 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human CD155/PVR Protein ELISA Data

  • Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.

Biotinylated Human CD155/PVR Protein

  • Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA.
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