Biotinylated Human CD155/PVR Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P15151
Source
DNA sequence encoding Human CD155/PVR including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD155/PVR is a protein consisting of amino acid residues [Trp21-Asn343] ,The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD155/PVR was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Methods
Biotinylated Human CD155/PVR Protein Tris-Bis PAGE
- Biotinylated Human CD155 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human CD155/PVR Protein SEC-HPLC
- The purity of Biotinylated Human CD155 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CD155/PVR Protein ELISA Data
- Immobilized Biotinylated Human CD155, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA.
Biotinylated Human CD155/PVR Protein
- Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA.
