2019 nCoV/COVID19 RBD Recombinant ,Biotinylated
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$1,200.00 – $2,500.00
Description
Accession
NCOVRBD
Source
DNA sequence encoding the COVID-19 spike RBD protein domain [319-541] accession #6VSB_A including a C-terminal His AVI tag was expressed in CHO Cells.
Molecular weight
Recombinant 2019-nCoV RBD protein is a protein consisting of 250 amino acid residues ,due to glycosylation migrates as an approximately 35kDa protein on SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Activity
The activity was determined by immobilized human ACE2 Protein binding with biotinylated RBD in a functional ELISA assay, the linear was determined to be 10-100 ng/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant COVID-19 RBD (Receptor Binding Domain) protein was lyophilized from 0.2 µm filtered PBS pH 7.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
