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Human Epiregulin Recombinant

Epiregulin

accession:  O14944
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 2700
Domain  :  EGF
Gene  :  EREG
Catalog no. :  RKO14944
Source:
Optimized DNA sequence encoding Human Epiregulin mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant Human Epiregulin, generated by the proteolytic removal of the signal peptide and propeptide, is a disulfide-linked monomer  protein.

Recombinant  Human Epiregulin consists of 50 amino acids and migrates as an approximately 6 kDa protein under reducing conditions.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 5 x 105 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MTAGRRMEML CAGRVPALLL CLGFHLLQAV  LSTTVIPSCI PGESSDNCTA LVQTEDNPRV 

        70         80         90        100        110        120 
AQVSITKCSS DMNGYCLHGQ CIYLVDMSQN YCRCEVGYTG VRCEHFFLTV HQPLSKEYVA 

       130        140        150        160        170 
LTVILIILFL ITVVGSTYYF CRWYRNRKSK EPKKEYERVT SGDPELPQV 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Human Epiregulin was lyophilized from a 0.2 μm filtered solution in PBS pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

EGF

Epiregulin, an epidermal growth factor family member, acts as a local signal mediator and shows dual biological activity, stimulating the proliferation of fibroblasts, hepatocytes, smooth muscle cells, and keratinocytes while inhibiting the growth of several tumor-derived epithelial cell lines. Epiregulin, a novel epidermal growth factor (EGF)-related growth factor first identified in NIH 3T3 cell conditioned medium , is one of seven known ligands for the epidermal growth factor receptor (EGFR). Epiregulin is synthesized as a transmembrane precursor before being proteolytically cleaved to release a 46- amino-acid active protein. Unlike other EGFR ligands, epiregulin shows dual biological activity; it stimulates proliferation of fibroblasts, hepatocytes, smooth muscle cells, and keratinocytes but inhibits growth of several tumor- derived epithelial cell lines. Epiregulin also shows more potent bioactivity in vitro than other EGF-like growth factors

Related Publications:
mechanics of EGF receptor/erbb2 kinase activation revealed by luciferase fragment complementation imaging
PNAS, Jan 2012; 109: 137 - 142.
heparin-binding EGF-like growth factor (hb-EGF) mediates 5-ht-induced insulin resistance through activation of EGF receptor-erk1/2-mtor pathway
Endocrinology, Jan 2012; 153: 56 - 68.
ectodomain shedding of hb-EGF: a potential target for cancer therapy
J. Biochem., Jan 2012; 151: 1 - 3.
the membrane-proximal intracellular domain of the epidermal growth factor receptor underlies negative cooperativity in ligand binding
J. Biol. Chem., Dec 2011; 286: 45146 - 45155.
protein-tyrosine phosphatase 1b modulates early endosome fusion and trafficking of met and epidermal growth factor receptors
J. Biol. Chem., Dec 2011; 286: 45000 - 45013.




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