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Rat basic Fibroblast Growth Factor Recombinant

bFGF

accession:  P13109
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 1100
Domain  :  HBGF/IL1
Gene  :  FGF2
Catalog no. :  RKP13109
Source:
Optimized DNA sequence encoding Rat basic  Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native Rat FGF basic is generated by the proteolytic removal of the signal peptide
and propeptide,the molecule has a calulated mass of 16 kDa.

Recombinant Rat bFGF is a disulfide-linked homodimeric protein consisting of 146 amino acid residue subunits, and migrates as an approximately 16 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>96%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) determined by  dose-dependent stimulation of  thymidine uptake by 3T3 cells expressing FGF receptors, was ofund to be <1 ng/ml.
 

 
Protein Sequence:
        10         20         30         40         50         60 
MAAGSITSLP ALPEDGGGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHVK 

        70         80         90        100        110        120 
LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTEEC FFFERLESNN YNTYRSRKYS 
       130        140        150 SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Rat Basic FGF was lyophilized from a 0.2 μm filtered PBS solution pH 7.5 .

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

bFGF

basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes . FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

Related Publications:
expression of bFGF and il-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 545.
p53 is necessary for bFGF-induced lens differentiation
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4369.
regulation of basic fibroblast growing factor (bFGF) by retinal glial (müller) cells under severe retinal ischemia
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2926.
natural history of progressive experimental cnv in the rabbit after sustained release of both vegf and bFGF within the supra-choroidal space
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2970.
keratocyte-associated mt1-mmp modulates bFGF-induced vegf expression
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 4967.




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Antibodies:
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