Description
Accession
P32971
Source
Optimized DNA sequence encoding Human CD30 Ligand including an N-terminal IgG1 Fc tag was expressed in HEK293 cells.
Molecular weight
Recombinant CD30 Ligand (CD30L) is a homodimer protein consisting of two 430 amino acid residue subunits, due to glycosylation migrates as an approximately 65kDa protein on SDS-PAGE under reducing conditions.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by the ability of immobilized recombinant human CD30L to bind Human CD30 with a linear range of 300-2000 pg/ml
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD30L is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant CD30 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Molecular function
