Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
O00220
Source
DNA sequence encoding Human TRAIL R1/DR4/TNFRSF10A including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human TRAIL R1/DR4/TNFRSF10A is a protein consisting of amino acid residues [Pro34-Asn239] ,The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human TRAIL R1/DR4/TNFRSF10A was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein Tris-Bis PAGE
- Biotinylated Human TRAIL R1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein SEC-HPLC
- The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein ELISA Data
- Immobilized Human TRAIL, No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31μg/ml determined by ELISA.
Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein SPR Data
- Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200).
