Biotinylated Human SIRP alpha/CD172a Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P78324
Source
DNA sequence encoding Human SIRP alpha/CD172a including a C-hFc-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human SIRP alpha/CD172a is a protein consisting of amino acid residues [Glu31-Arg370] ,The protein has a predicted MW of 66.1 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human SIRP alpha/CD172a was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human SIRP alpha/CD172a Protein Tris-Bis PAGE
- Biotinylated Human SIRP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human SIRP alpha/CD172a Protein SEC-HPLC
- The purity of Biotinylated Human SIRP alpha is greater than 95% as determined by SEC-HPLC.
Biotinylated Human SIRP alpha/CD172a Protein ELISA Data
- Immobilized Human CD47, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
