Biotinylated Human Notch 1 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P46531
Source
DNA sequence encoding Human Notch 1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Notch 1 is a protein consisting of amino acid residues [Ala19-Gln526] ,The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Notch 1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P62988
Interactor
Interactor
Q06330
Interactor
Interactor
Q92585
Interactor
Q06330
Interactor
Q8VBV4
Interactor
Q969H0
Biological Process
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Biotinylated Human Notch 1 Protein Tris-Bis PAGE
- Biotinylated Human Notch 1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human Notch 1 Protein SEC-HPLC
- The purity of Biotinylated Human Notch 1 is greater than 95% as determined by SEC-HPLC.
